Tuesday, April 21, 2020
What Is The Nature And Substance Of Organisational Culture To What Ex
What Is The Nature And Substance Of Organisational Culture? To What Extent Can It Be Changed? Culture, the acquired knowledge that people use to interpret experience and generate social behaviour (Spradley, 1979, p. 5), provides people with a way of seeing the world. It categorizes, encodes, and otherwise defines the world in which they live. Whenever people learn a culture, they are to some extent imprisoned without knowing it. Anthropologists talk of this as being culture bound--i.e., living inside a particular reality. References to culture have long abounded in professional literature. However, it is only fairly recently that the literature shows references to culture as a lens through which to interpret and understand organizations, their customers, and the working relationships therein (Lee Shaughnessy, 1988). The cultural analysis of organizations, therefore, is the use of organizational culture as a lens through which to examine what is going on in an organization. Management theory in the 1980s underwent a sea of change in its realization that an understanding of an organization's culture(s) could be a major step on the road to changing or controlling the direction of that organization. There are both positive and negative sides to how an understanding of culture can be used within an organization. For instance, Edgar Schein (1992) considers the process of creating culture and management to be the essence of leadership, while Gideon Kunda (1992) describes a culture which embodies both the implicit and explicit rules and behaviour of a particular group of people and the conscious efforts of management to engineer the culture to its own goals. There is a fundamental distinction between those who think of culture as a metaphor (Morgan, 1986) and those who see culture as an objective entity. (Gold 1982) Metaphors allow us to understand organisations in terms of other complex entities such as the machine and the organism. By observing the similarities, scholars attempt to explain the essence of human organisations. The dangers of such an approach is distinguishing when the metaphor is no longer valid. That is why most commentators have chosen to think of culture as an objective entity. This view have ranged from viewing the organisation literally as a culture with all features of an organisations including its systems, policies procedures and processes as elements of its cultural life (Paconowsky & O'Donnell-Trujillo 1982) to suggesting that culture is best thought of as a set of psychological predispositions, called ?basic assumptions', that members of an organisation possess that leads them to think and act in a certain way .(Schein 1985). The former view presents problems in using the concept to explain other aspects of organisational activity. Indeed if everything is culture, this view becomes indistinguishable from the view that culture is a metaphor. This leaves us with Schein's view of culture as an essentially cognitive phenomena that resides in the psychology of organisational participants, with the acknowledgement that patterns of behaviour are equally important (Eldridge & Crombie 1974) The contents of an organisational culture has several levels. At the basic and superficial level, it takes the form of artefacts like stories, jokes metaphors and symbols. Examples of artefacts would be Material objects like mission statements, corporate logos , Physical layout of the office space etc. At a deeper level, culture takes the form of values beliefs and attitudes. Values determine what people ought to do while beliefs are what people think is or is not true. In practice, beliefs and values are often hard to distinguish, because beliefs frequently involve values. Moreover, there is considerable merit to viewing values as a particular sort of belief. (Rokeach 1973: 5) Attitudes connect beliefs and values with feelings. An attitude is a learned predisposition to respond consistently to a particular thing or idea. Attitudes are developed over time and unlike opinions, are held relatively consistently. At the deepest level, culture in an organisation takes the form of basic assumptions, a solution to an identifiable problem that is taken for granted. These are implicit, deep-rooted assumptions that people share, and which guides their perception, feelings and emotions about things. (Schein, 1981) Basic assumptions are held unconsciously and are very difficult to surface. Basic assumptions are by definition neither confront able nor debatable. Basic assumptions are also
Monday, March 16, 2020
Mental Imagery Its Nature and Use with Regard to Motor Performance essays
Mental Imagery Its Nature and Use with Regard to Motor Performance essays Mental imagery is a technique that has been increasingly used to enhance motor performance, especially as this relates to fitness and sports. According to Annie Plessinger, mental imagery, visualization or mental rehearsal, is an experience in the mind that resembles the actual experience without any physical activity or stimuli. Another, and perhaps clearer, definition is that mental imagery involves the mental repetition of a certain skill or movement, without the accompanying physical movement ("The Use of Mental Imagery in Soccer"). Basically the definition of the term entails that in motor performance, mental imagery entails rehearsal using the mind only, without any physical involvement. Mental imagery thus means to imagine movement without carrying it out (Plessinger). In fitness and sports, this means visualizing a certain skill or movement in the mind. This can help the mind to become used to the skill in order to better prepare the body for physical performance. David Yukelson emphasizes the importance of the appropriate feeling that should accompany mental imagery. The image in the mind is then accompanied by the desired feeling of for example confidence and control. Various terms are used in association with mental imagery as applied to fitness and sports. One definition ("The Use of Mental Imagery in Soccer") for example includes a division of the term into external and internal mental imagery. External imagery occurs when a soccer player for example visualizes himself as if he is watching a separate person performing the actions. Internal imagery on the other hand occurs with the player imagining the actions performed by his own body. Yukelson uses the term "feelization" to denote the sensation created by internal imagery. The player feels as it were the actions being imagined. Different people have differen ...
Friday, February 28, 2020
Introduction to Accounting and finance -- Economics, Finance and Assignment - 3
Introduction to Accounting and finance -- Economics, Finance and Management - Assignment Example They are very disturbed with months like September recording a high sales volume and November recording a low sales volume. This could prove to be an issue for the working capital in the long run and the concept of going concern. (Fields. E., 2002). It would be better if the managerââ¬â¢s focus on achieving uniform sales targets every month so that the working capital available could be more consistent giving way for better planning. 3. If the company would continue with bulk purchases and not go for investing further in machinery, there could be a possibility of maintaining obsolete machinery. As it is, the company has only machinery worth à £25,000 and further non-investment may prove to be a hindrance to exploring newer avenues of market creation. (Clemmer. J., 1995). However, it needs to understand that its retained earnings which represent the profit over the years are quite high. They almost represent double of the ownerââ¬â¢s equity. This means that the companyââ¬â¢s overall profitability is commendable. Cash, on the other hand, represents the revenue or liability of that company. Revenue is generated by sales of that company. This is explained in the trading account of the firm. The other way to generate cash is to take up a liability. This is shown in the balance sheet of the firm. In the case of Hide to seek ltd there is a negative liquidity possibility for two months. This is because the company is planning to purchase materials in bulk. These materials are further sold in the coming months. There is no incidence of purchases in the next six months. Bulk purchases ensure discounted price. That means, while cash reserves are decreasing for two months, there is an indirect increase in profits in the next 3 months. This is the strategy to withhold benefits to gain higher of them in the future. This has to be given as an explanation in the footnotes to the financial statements. (Lander. P.G., 2004). If Hidetoseek ltd decides to investigate
Wednesday, February 12, 2020
The Gathering Storm and Global War (US History) Assignment
The Gathering Storm and Global War (US History) - Assignment Example The Axis powers wanted to rule the world. If not stopped by the combined efforts of the Allies, this could have been a reality. Without the U.S.ââ¬â¢s support, the English, Russians, and other Allies might have failed to defeat the Axis powers. 2) JAPANESE INCARCERATION-Should the survivors of the Japanese prison camps have been given a $20,000 reparations payment from 1988 on, or was their imprisonment just the fortunes of war? Explain. Yes. Japanese prison camps should have been given $20,000 in reparations, not only for their economic and material losses, but also for the emotional turmoil at the loss of security of being an American citizen. If we did not issue reparations, then we would have been worse than the NAZIS. We are supposed to be good guys, although America sometimes makes mistakes. These mistakes need to be acknowledged and paid for. Japanese prison camps were not only a mistake, but wrong as well. 3) ATOMIC BOMB-Was there any better option for Pres. Harry Truman to end WW2 than dropping two atomic bombs on Japan? Explain. No. The Japanese had a different perspective about death. Our goal was to protect as many American lives as possible. The Japanese did not mind dying in kamikaze attacks as long as Americans also died. If the atomic bomb had not been used, the war could have lasted indefinitely. This might have caused more causalities, American and Japanese, than the ones that died in Hiroshima or Nagasaki
Friday, January 31, 2020
Jazz Essay Example | Topics and Well Written Essays - 1250 words - 2
Jazz - Essay Example Miles Davis one of the most famous jazz players worked on the modern jazz musical concepts that have helped to grow this genre to a next level. The very first jazz melody ââ¬Å"Agitationâ⬠gives the listener a very exotic feeling by using his trumpet. It combines the different aspects from the musical perspectives. The song follows quick transitions with specific background sounds. The background music keeps the flow of the music steady and continuous. The trumpet is used throughout the music owing to its main emphasis on the intensity to the certain point it shows. Miles Davis improvisation in this specific type of Jazz know as Modal Jazz music has helped specifically to stretch out the harmony of the music into certain parts which makes the it more systematic and helps the music to proceed in an organized manner. Miles Davis stretched out certain improvements in different types of Jazz music, such as bebop, hard bop, cool jazz, modal jazz and jazz fusion. This specific piece from Miles Davis joins in with different instruments to give the feel of the environment producing a story of its sort. This modal jazz requires knowledge of musical nodes beforehand. Miles Davis, the most well-known protagonist of Jazz Music history had been on top of creating creative method for Modal Jazz. The video specifically has piano to create the specific background rhyme to the music that follows throughout the length of the video. Throughout the video there are specific changes in the chords which at times follow a certain transition and after a break follow another set of harmonies. In its historical aspect, this piece shows the jazz music that followed up in the 60ââ¬â¢s. Miles Davis, who was a pro trumpeter, performed his music based either solo or like in the video, along with different instruments such as French horn, Tuba, Wood winds. The video reflects on the charismatic methods of attaining the attention by progressive music. This video stretches
Thursday, January 23, 2020
leadership :: essays research papers
There are two broad approaches to seeking to shape values in society. One is by being "salt and light" in the world (Mt 5:13,14), where by living distinctively Christian lifestyles we seek to influence those around us, and through this the values, decisions and priorities adopted by our communities. The other approach is to confront a particular value, decision or priority which the Christian community feels is out of place, and this may require a more structured and focused campaign. This page offers a six step approach to run such a campaign, whilst click here to view some thoughts on being salt and light. The six steps below seek to help a leader put some structure around an influencing campaign. This may be related to a specific proposal within a community, or more generally trying to influence the values that the community adopts. This community may be a geographic community : neighbourhood, town or even country, or it may be another form of network eg work or interest related. 1. Know what you're trying to achieve. Ã · Write down some specific aims for your influencing. If possible, make these aims measurable. Ã · These may be related to a specific issue that has arisen within the community, or it may be seeking to change more general values and behaviours. Don't overload with issues - it's better to focus on one or two and achieve something, than make little progress against a broad agenda 2. Decide on your audience and message. Ã · Who are the people who hold the key to achieving your objectives? This may include those with formal responsibility (councillors and MPs) , those who can influence them (respected thought leaders and influential organizations) and influential channels such as the media. Ã · What is the action you want them to take, and therefore, what is the message that you need to deliver to them to persuade them to take such action? Can you write it down - physically writing the message down is very worthwhile as it ensures that there is sufficient clarity of thinking. Ã · The same message can be received very differently when delivered by different people or organizations. Taking the time to identify the most credible and capable people to deliver the message is important. In some cases, you may want to focus on people with specific skills, knowledge or position who can speak with authority.
Wednesday, January 15, 2020
Quantification of Proteins in Solution by Spectrophotometer Essay
Introduction: Absorption spectroscopy is a common method for finding the concentration of proteins or protein complexes in a solution. Proteins absorb light at specific wavelengths and can be defined by the equation A = log (Io/I). This equation states that an absorbance at a specific wavelength, A is equal to the log of the ratio of incident light intensity (Io), to transmitted light intensity (I). A spectrophotometer can be used quantitatively and qualitatively. A spectrophotometer is used qualitatively to obtain an absorption spectrum, which can be obtained by plotting the absorbance values, over the range of wavelengths tested for the solution. This helps to find out the suitable wavelength that the compound absorbs maximum. And the spectrophotometer is used quantitatively by using the Beer-Lambert Law; Log [ Io/I] = A = à ¾cl, where à ¾ is the molar extinction coefficient (unit = Lmol-1cm-1), helps to define the absorbance of the protein, c is the concentration of the substance (mol liter-1), and l is the path length of the light (unit = cm) through the medium. Log [Io/I] is called optical density or absorbance of the substance, and does not have units. Also, an absorption spectrum is created, which deals with absorption and wavelength (nm) of light used, with which ââ¬Å"maximum absorptionâ⬠is observed. Maximum absorption is when most of the solution particles are absorbed, and this happens at a specific wavelength. Since the Beer Lambert law is useful only for a range of wavelengths, it is not applicable to all protein solutions. In this experiment, an absolute standard was calculated using BSA, so that the concentrations of the other unknown protein solutions can be determined (Lambert et.al, 2011).The different assays used for this protein quantification were Lowry, Bradford (Coomassie Blue) and UV direct. Protein assays help to determine the amount of desired particle present (Srivastava, 2008). The aim of this lab is to understand the various aspects of spectrophotometry and its applications in biochemistry, such as quanitification of protein solutions. Methods: (Carleton University, 2012) The steps were followed without any changes made. Results: Figure1. Absorption spectrum of 6Ãâ"10-5M p-np solution in 0.02M NaOH, for wavelength range between 330-800nm using a Novaspec spectrophotometer. Sample Calculation: c = 6Ãâ"10-5M l = 1cm A = 1.166 The Beer Lambert equation is A = à cl Rearranged, à = A/cl à = 1.166/(6Ãâ"10-5)*1 à = 1.94Ãâ"104 L mol-1 cm-1 Table1. Values of extinction coefficient (Lmol-1cm-1) determined using Beer-Lambert Law. Figure2. Absolute standard curve obtained for BSA test protein solution with the 3 different assays tested (Lowry, Coomassie Blue, UV). Table 2. Absorbance values recorded for different protein dilutions (2X, 5X, 10X) for the three assays used, namely Lowry, Coomassie Blue and UV direct. Sample Calculation for BSA stock protein: * Lowry Equation of line from Fig2; y = -5Ãâ"10-7Ãâ"2 + 0.0016x + 0.038 For 5 fold; y = 5 * 0.44 = 2.20 Substituting in equation; 2.20 = 5Ãâ"10-7Ãâ"2 + 0.0016x + 0.038 X1 = 1600 à µg/ml = 1.6mg/ml For 10 fold; y = 10 * 0.23 = 2.30 Substituting in equation; 2.30 = 5Ãâ"10-7Ãâ"2 + 0.0016x + 0.038 X2 = 1600 à µg/ml = 1.6mg/ml (X1 + x2)/2 = 1.6mg/ml * Coomassie Blue Equation of line from fig2; y = -7Ãâ"10-7Ãâ"2 + 0.002x + 0.0219 For 5 fold; y = 5 * 0.36 = 1.80 Substituting in equation; 1.80 = -7Ãâ"10-7Ãâ"2 + 0.002x + 0.0219 X1 = 1428.57 à µg/ml = 1.4mg/ml For 10 fold; y = 10 * 0.20 = 2.00 Substituting in equation; 2.00 = -7Ãâ"10-7Ãâ"2 + 0.002x + 0.0219 X2 = 1428.57 à µg/ml = 1.4mg/ml (x1+ x2)/2 = 1.4mg/ml * UV direct Equation of line from fig 2; y = 0.0006x + 0.0175 For 2 fold; y = 2 * 0.42 = 0.84 Substituting in equation; 0.84 = 0.0006x + 0.0175 X1 = 1374.16 à µg/ml = 1.4mg/ml For 5 fold; y = 5 * 0.15 = 0.75 Substituting in equation; 0.75 = 0.0006x + 0.0175 X2 = 1179.16 à µg/ml = 1.2mg/ml (x1 + x2)/2 = 1.3mg/ml Discussion: Figure 1 shows the absorption spectrum of stock solution (6Ãâ"10-5M), p-nitrophenol and 0.02M NaOH, and from the graph it can be inferred that 400nm is the wavelength of maximum absorption because absorption is noted to be the highest at this point. Absorbance is noted to increase when wavelength increases till it reaches the point of maximum absorption, after which it decreases till it nearly reaches zero. It is best to consider wavelength of maximum absorption because stronger the intensity, the more accurate will be the readings for absorbance. As seen from table 1, the path lengths remain the same as the cuvettes used were of the same size. The Beer-Lambert Law states that Abs = à .c.l, where à = molar extinction coefficient, c = concentration of protein solution, and l = path length of light through medium. Thus, it is noted that absorbance and path length share a directly proportional relationship, i.e. if path length increases, absorbance increases as well. It was clear ly observed in the wide and narrow test-tubes, that as the path length was doubled, the absorbance value doubled too (Srivastava, 2008). Also, from the same equation, it can be determined that absorbance and concentration share a directly proportional relationship meaning that as the concentration decreases, it directly affects the absorbance value obtained, and this value decreases too. Thus, as seen for the four cuvettes tested (in Table 1) as the concentration is halved in every cuvette, the absorbance value is halved correspondingly as well. It is known that the Beer-Lambert law says absorbance is proportional to number of absorbing molecules, and that this is valid for a variety of compounds over a wide range of concentrations. But even as the molar extinction coefficient is seen to be attributed to wavelength, it is true only for monochromatic light (Lambert et.al, 2011). The relationship can be stated as ââ¬Å"à is a measure of the amount of light absorbed per unit concentrationâ⬠. Molar extinction coefficient is a constant for a particular substance, therefore according to the Beer-Lambert Law it is expected that if the concentration of the solution is halved so is the absorbance. A compound with a high molar extinction is very effective at absorbing light (of the appropriate wavelength), and hence low concentrations of a compound with a high molar extinction can be easily detected. In the values determined (Table 1), the experimental values are in accordance with the theoretical statement except for one cuvette. The cuvette no.3 with à = 1.8Ãâ"10-4 L mol-1cm-1 does not agree with the trend. Thus it can be deduced that due to experimental error, the à value is inaccurate. Also, from the equation it is understood that à and path-length are inversely proportional as well (i.e. à = Abs/cl) that means that as path-length increases, à decreases, assuming that the concentration is kept constant. But the experimental values do not agree with this statement, because it is seen that as the path-length increases so does the molar extinction coefficient, à . Biochemical methods are applied for to determine protein concentration in solutions. Many techniques are less used because they have limitations such as reduced sensitivity, time available for the assay, or they are highly specific about the amino acids in the protein solution being tested. But for e very protein, the component amino acids are different, so there is no single assay that can be used for quantification of all proteins. The absorbance assays use the method of testing the intensity of the color produced by the protein solutions when chemical reagents are added to it. A ââ¬Å"standard proteinâ⬠whose concentration is known, is treated using the same chemical reagents and thus an absolute standard curve is obtained (Boyer, 2000). In this experiment, the standard used was Bovine Serum Albumin (BSA). Development of color is significantly better in BSA than any other protein, and this makes it one of the most preferred test solutions for quantification of proteins (Antharavally et.al, 2008). Hence figure 2 is obtained by performing the three suitable assays on BSA to produce a standard curve, also it can be noted that only the UV direct gave a straight line passing through zero, whereas the Lowry and Coomassie Blue gave curved lines, passing through zero. Table 2 shows the absorbance values recorded, for different dilutions of the test protein in three different assays. With the help of the values obtained in Table 2, and with the equations obtained from Figure 2, the concentration of protein (mg/ml) was calculated and presented in Table 3. Since all the values in Table 3 were deduced from the equation of standard curve BSA, it is considered as the absolute standard, and the other test protein solutions are known as the relative standards. Using the values from Table 3, taking BSA as the absolute standard, the almost actual concentration of the protein (mg/ml) can be concluded, and they are 1.6 (mg/ml) for Lowry assay, 1.4 (mg/ml) for Coomassie Blue and 1.3 (mg/ml) for UV direct. For Lowry assay, the concentration value for all test proteins was 1.6 mg/ml, which must mean that the value obtained is accurate. For Coomassie blue, BSA and Hemoglobin were the same (1.4mg/ml), Ovalbumin and Lysozyme had similar values of 1.9mg/ml, and 1.8mg/ml respectively, whereas Gamma globulin showed 2.5mg/ml. The value for Gamma globulin is off because of experimental error, of spilling some of the contents from the cuvette while transferring it to the spectrophotometer for calibration. For UV direct, BSA and Ovalbumin have similar readings (1.3mg/ml and 1.5mg/ml respectively), Gamma globulin is 2.5mg/ml, but Lysozyme is 5.9mg/ml and Hemoglobin is 3.8mg/ml. The reason for this could be due to the fact that UV direct helps to identify the presence of aromatic compounds indicating that Lysozyme and Hemoglobin contain aromatic compounds present in them. The Lowry protein assay is the most common and one of the more sensitive, but it is time consuming, on the other hand Coomassie blue (the Bradford assay) is much more sensitive as compared to Lowry, and requires less time too. They both show change of color with proteins. As for UV direct method, it is one of the faster methods too, and it is helpful to identify aromatic compounds because aromatic residues absorb 280nm light (Boyer, 2000). The Lowry procedure can detect protein levels as low as 5à µg (Boyer, 2000). It depends on the color development by the reagent Folin-Ciocalteu. Peptide bonds are formed under alkaline Cu2+ conditions and reduced from Folin-Ciocalteu phosphomolybdate-phosphotungsten by aromatic amino acids (tyrosine and tryptophan) to heteropolymolybdenum blue. The standard curve obtained with BSA helps to determine concentration of unknown protein solutions (Antharapally et.al, 2008). In the case of Coomassie blue, it is more efficient than Lowry because even though there is variation with different proteins, there is very less interference by non protein components (Borley, 2000). Therefore, according to literature, Coomassie Blue is the most preferred protein assay but this contrasts the experimental inferences, because through experimental procedure it was seen that Lowry method gave the most accurate and precise results. With this experiment, the method to quantify unknown protein concentrations has been understood. Also, that this process must be performed carefully to avoid irrational experimental errors. References: Antharavally B.S, Bell P.A, Haney P, Mallia K.A, Rangaraj P. 2008. Quantitation of proteins using a dyeââ¬âmetal-based colorimetric protein assay. Analytical Biochemistry. 385; 342-245. Boyer R, 2000. Modern Experimental Biochemistry, third edition. Addison-Wesley Longman, Inc. USA. (41-45). Lambert J.B, Gronert S, Lightner D.A, Shurvell H.F, 2011. Organic Structural Spectroscopy, second edition. Pearson Education, Inc, New Jersey. (401, 404) Srivastava M.L, 2008. Bioanalytical Techniques. Alpha Science International, Ltd. Oxford, UK. (58,118)
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